The characteristics of the binding of 12‐alpha‐[3H]‐digoxin to the membranes of intact human erythrocytes: relevance to digoxin therapy.
Ford A., Aronson J., Grahame‐Smith D., Rose J.
1 The characteristics of the binding of 12‐alpha‐[3H]‐digoxin to the membranes of intact human erythrocytes are described. 2 Only one class of binding site can be demonstrated. Binding is time‐ and temperature‐ dependent, saturable and slowly reversible; it is inhibited by other cardiac glycosides and by potassium. 3 Pre‐incubation with unlabelled digoxin reduces the subsequent binding of 12‐alpha‐[3H]‐digoxin in stoichiometric fashion. 4 The possible application of the measurement of the binding of 12‐alpha‐[3H]‐digoxin to the study of biochemical pharmacological events occurring during digoxin therapy is discussed. 1979 The British Pharmacological Society