From long range mapping to sequence-ready contigs on human chromosome 6
Mungall AJ., Humphray SJ., Ranby SA., Edwards CA., Heathcott RW., Clee CM., Holloway E., Peck AI., Harrison P., Green LD., Butler AP., Langford CF., Gwilliam R., Huckle EJ., Baron L., Smith A., Leversha MA., Ramsey YH., Clegg SM., Rice CM., Maslen GL., Hunt SE., Scott CE., Soderlund CA., Theaker AJ., Carter NP., Ross MT., Deloukas P., Bentley DR., Dunham I.
Our aim is to construct physical clone maps covering those regions of chromosome 6 that are not currently extensively mapped, and use these to determine the DNA sequence of the whole chromosome. The strategy we are following involves establishing a high density framework map of the order of 15 markers per Megabase using radiation hybrid (RH) mapping. The markers are then used to identify large-insert genomic bacterial clones covering the chromosome, which are assembled into sequence-ready contigs by restriction enzyme fingerprinting and sequence tagged site (STS) content analysis. Contig gap closure is performed by walking experiments using STSs developed from the end sequences of the clone inserts. © 1997 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.